Background: Three extracts were prepared from the leaves of Accacia salicina; ethyl acetate (EA), chloroform (Chl)\r\nand petroleum ether (PE) extracts and was designed to examine antimutagenic, antioxidant potenty and oxidative\r\nDNA damage protecting activity.\r\nMethods: Antioxidant activity of A. salicina extracts was determined by the ability of each extract to protect\r\nagainst plasmid DNA strand scission induced by hydroxyl radicals. An assay for the ability of these extracts to\r\nprevent mutations induced by various oxidants in Salmonella typhimurium TA102 and TA 104 strains was\r\nconducted. In addition, nonenzymatic methods were employed to evaluate anti-oxidative effects of tested extracts.\r\nResults: These extracts from leaf parts of A. salicina showed no mutagenicity either with or without the metabolic\r\nenzyme preparation (S9). The highest protections against methylmethanesulfonate induced mutagenicity were\r\nobserved with all extracts and especially chloroform extract. This extract exhibited the highest inhibitiory level of\r\nthe Ames response induced by the indirect mutagen 2- aminoanthracene. All extracts exhibited the highest ability\r\nto protect plasmid DNA against hydroxyl radicals induced DNA damages. The ethyl acetate (EA) and chloroform\r\n(Chl) extracts showed with high TEAC values radical of 0.95 and 0.81 mM respectively, against the ABTS.+.\r\nConclusion: The present study revealed the antimutagenic and antioxidant potenty of plant extract from Accacia\r\nsalicina leaves.
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